Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1990-07-19
1993-07-06
Chereskin, Che S.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
800205, 4353201, 935 30, 935 35, 935 64, C12N 1563, C12N 1582, A01H 400
Patent
active
052253414
ABSTRACT:
This invention relates to methods for producing transgenic plants that contain a gene of interest and that are free of foreign ancillary nucleic acids. These methods allow for the production of plants which thus contain a desired gene, but which are free of vector sequences and/or marker sequences used to transform the plant. The method of transforming such plants calls for transforming the plants with a gene of interest by introduction of the gene on a DNA construct comprising a transposon and foreign ancillary nucleic acids; crossing the transformed plant through self-crossing or with another plant to obtain F.sub.1 or more removed generation progeny; and utilizing a means for selecting those progeny that carry the gene of interest and are free of the ancillary nucleic acids. Such progeny may be detected biochemically, by Southern hybridization, through the use of polymerase chain reaction procedures and other methods available in the art.
REFERENCES:
patent: 4732856 (1988-03-01), Federoff
Masterson et al (1989) Mol. Gen. Genet. 219: 461-466.
Belzile, et al (Sep. 1989) Genetics 123: 181-189.
Yoder, J. I. et al., "Ac Transposition in Transgenic Tomato Plants," Mol. Gen. Genet. 213:291-296 (1988).
Belzile, F. et al., "Sexual Transmission of Transposed Activator Elements in Transgenic Tomatoes," Genetics 123:181-189 (1989).
Lassner, M. W. et al., "Genetic Transactivation of Dissociation Elements in Transgenic Tomato Plants," Mol. Gen. Genet. 218:25-32 (1989).
Yoder, J. I. et al., "A Single Copy Ds Insertion Library in Tomato," in Horticulture Biotechnology, Bennett, O'Neill, (eds.) 1990, Wiley Press, Inc. pp. 85-94.
Baker, B. et al., "Phenotypic Assay for Excision of the Maize Controlling Element Ac in Tobacco,", EMBO J. 6:1547-1554 (1987).
Weide, R. et al., "A Simple, Nondestructive Spraying Assay for the Detection of an Active Kanamycin Resistance Gene in Transgenic Tomato Plants," Theor. Appl. Genet. 78:169-172 (1989).
Lassner Michael W.
Yoder John I.
Chereskin Che S.
The Regents of the University of California
LandOfFree
Biologically safe plant transformation system using a Ds transpo does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Biologically safe plant transformation system using a Ds transpo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Biologically safe plant transformation system using a Ds transpo will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1689027