Biologically pure Bacillus badius ferm BP-4493 having deodorizin

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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43525231, 435832, C12N 100, C12N 120

Patent

active

058112871

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BRIEF SUMMARY
FIELD OF THE INVENTION

The present invention relates to a substantially pure cultured microorganism of Bacillus badius MA001 (FERM BP-4493) strain having deodorant and sporogenic properties, a microorganism composition containing the substantially pure cultured microorganism of Bacillus badius MA001 strain having deodorant and sporogenic properties as the active ingredient, and a deodorizing method using the said miroorganism composition.


PRIOR ARTS

A deodorizing method by decomposing offensive odor sources by the use of specific microorganisms, i.e. microbial deodorizing method, has been developed by means of the recent biotechnology. A deodorizing process in the microbial deodorizing method is based on the decomposition of odorous substance from offensive odor sources. At present, soil deodorizing method is developed as an application of microbial deodorizing method. This is a deodorizing system for decomposing offensive odorous substance by an action of soil microorganisms in soil after discharging the collected odor which is blown into pipes set in the soil.
Microbial deodorizing method in which odorous gas is blown into a column packed with immobilized microbial carrier, has also been developed. Examples of the carrier are sawdust, peat moss and rock wool. In the operation of these deodorizing methods, there are so many problems, for example constant water spray and pH adjustment are essential and require the high cost on maintenance of the soil and carrier. Catching the offensive odor is practically quite troublesome and gathering offensive odorous gas requires high cost which necessitate the construction of large scale plants. Furthermore treatment capacity has to be limited due to high loading in the treatment.
Microorganisms which act as microbial deodorant are generally soil bacteria, therefore deodorizing effects are different depending on the soil used and type of odor substances. For obtaining practically sufficient deodorizing effects, one to several months for adaptation of offensive odor substances are necessary and in that case constant stable operation can not be expected. Adaptation by offensive odor result in the growth of specific soil bacteria and generation of ammonia. In this case, bacteria which do not generate excess ammonia are essentially required.
Mechanisms of deodorizing action by soil bacteria are mostly unknown, and are generally recognized as a cleaning up action of soil bacteria. Few reports are known in relation to the deodorizing action of single or multiple microorganisms. Reports on the studies in view of microbial action are not so many. Major studies on microorganisms which decompose organic sewage and offensive odorous substance are conducted on the sewage treatment by activation sludge method. Studies on the properties of microorganisms which decompose offensive odorous substance and decomposition process are in progress.
Deodorant containing specific microorganism, especially in the case that offensive odorous composition is sulfur compound, is reported, for example Letters, 34 (1986) 13-19! and Pseudomonas which decomposes hydrogen sulfide (Japan. Pat. Unexam. Publ. No. 4-262778). Acinetobacter calcoacetius which can decompose indole and skatole and a microbial decomposition of indole and skatole are known (Japan. Pat. Unexam. Publ. No. 2-53482). Decomposing offensive odorous substance by use of single microorganisms are limited due to practically complex offensive odor and the deodorizing effects can not be expected. These microorganisms in the deodorant are not stable for long term, and maintaining vaiable counts of microorganisms in the offensive odor source are difficult.
Microorganisms which decompose indole and skatole such as Bacillus sp. ID246 and a biological decomposition of indole and skatole have been known (Japan. Pat. Unexam. Publ. No. 2-53481). A microorganism belonging to Bacillus has an activity for decomposing specific offensive odor such as indole and skatole, however deodorized offensive odor is limited and deodorizing activity is not so

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