Biologically functional molecular chimeras

Details Biologically functional molecular chimeras Biologically functional molecular chimeras

1984-04-20

1988-04-26

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 91, 435320, 4352402, 435253, 935 27, 935 31, 935 32, C12N 1500, C12N 500, C12N 120, C12N 100, C12P 1934

Patent

active

047404701

ABSTRACT:
Method and compositions are provided for replication and expression of exogenous genes in microorganisms. Plasmids or virus DNA are cleaved to provide linear DNA having ligatable termini, which are bound to a gene having complementary termini, to provide a biologically functional replicon with a desired phenotypical property. The replicon is inserted into a microorganism cell by transformation. Isolation of the transformants provides cells for replication and expression of the DNA molecules present in the modified plasmid. The method provides a convenient and efficient way to introduce genetic capability into microorganisms for the production of nucleic acids and proteins, such as medically or commercially useful enzymes, which may have direct usefulness, or may find expression in the production of drugs, such as hormones, antibiotics, or the like, fixation of nitrogen, fermentation, utilization of specific feedstocks, or the like.
The invention was supported by generous grants of NIH, NSF and the American Cancer Society.

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