Biologically active membrane material

Details Biologically active membrane material Biologically active membrane material

1976-05-10

1978-01-03

Naff, David M.

Textiles: fiber preparation

195 63, 1951035R, 1951035A, 1951035Y, 260 6, 260112R, 424 2, C07G 700, C07G 702, G01N 3114

Patent

active

040665127

ABSTRACT:
A composite membrane structure for immobilizing biologically active materials, such as enzymes, is formed by coating a microporous polymeric membrane with a thin layer of an inert proteinaceous material, such as zein or collagen, so that the resultant coated membrane retains intercommunicating capillary pores that extend through its structure. Immobilization of a biologically active material is carried out by contacting the coated membrane with the biologically active material in solution and drying. Biologically active materials immobilized on the membrane can be used to perform biochemical reactions and are useful in carrying out tests for glucose and uric acid.

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Olson, et al., Immobilized Enzymes in Food and Microbial Processes, Plenum Press N.Y. 8/1974, (pp. 157-168, 176-178 & 181-182).
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Thong, et al., Observations on the Activity of Enzymes after Filtration on (and through) a Nitrocellulose Membrane, Biochemical and Biophysical Res. Comm., vol. 31, 1968 (pp. 1-8).
Wheeler, et al., Properties of two Phosphatases, Attached to Insoluble Cellulose Matrices; Biochim. Biophys. Acta. vol. 191, 1969 (pp. 187-189).
Zaborsky, O., Immobilized Enzymes, CRC Press, Chemical Rubber Co., Cleveland, Ohio, 1973 (pp. 73, 80, 83, 130-132, 151 & 158).

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