Biologically active fragment of bacillus stearothermophilus DNA

Details Biologically active fragment of bacillus stearothermophilus DNA Biologically active fragment of bacillus stearothermophilus DNA

1996-04-17

1998-11-03

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435194, 4353201, 4352523, 435325, 435419, 536 232, C12N 912, C12N 1554, C12P 1934

Patent

active

058307141

ABSTRACT:
The present invention is directed to an isolated and purified DNA encoding a biologically active fragment of a thermostable, full length DNA polymerase I enzyme of Bacillus stearothermophilus. More particularly, the invention is directed to a DNA encoding an approximately 66,000 dalton DNA polymerase that lacks 273 amino acids from the N-terminus of the approximately 96,000 dalton B. stearothermophilus DNA polymerase I, and to the protein encoded thereby which has been designated the B. stearothermophilus DNA polymerase I exo- fragment. The enzyme fragments are useful in DNA sequencing, cDNA preparations, thermophilic Strand Displacement Amplification and other molecular biology applications.

REFERENCES:
patent: 5436326 (1995-07-01), Ishino et al.
Phang et al., Gene, (1995) vol. 163:65-68.
Kabov et al., J. Bact., Jan. 1981, 145(1):21-26.

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