Multicellular living organisms and unmodified parts thereof and – Nonhuman animal
Patent
1995-04-07
1999-03-02
Campbell, Bruce R.
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
800DIG1, 424 92, 435975, 436815, C12N 500, C12N 1500, G01N 3353
Patent
active
058773983
ABSTRACT:
A method and kit for detecting toxins and pollutants is disclosed. The kit includes a transgenic organism having a stress-inducible control region linked to a gene encoding a detectable protein wherein said control region regulates the expression of said detectable protein; exposing said organism to said sample; and determining the amount of detectable protein produced. Exposure of this organism to a toxin or pollutant induces the production of the detectable protein which can be easily measured. This invention provides a rapid and reliable system for testing samples for the presence of toxins or pollutants.
REFERENCES:
patent: 4345026 (1982-08-01), Lew
patent: 4444891 (1984-04-01), Miwa et al.
Stringham and Candido, "Use of Transgenic Strains of The Nematode Caenorhabditie elegans as Biological Monitors of Environmental Stress," in SETAC 92, Abstracts of the 13th Annual Meeting of the Society of Environmental Toxicology and Chemistry, Nov. 8-12, Cincinnati, 1992, p. 10, Abstract No. 91.
Williams et al., "Using the Nematode Caenorhabditis elegans to predict mammalian acute Iethality to Metallic Salts," Toxic and Industrial Health 4: 469-478, 1988.
Williams et al., "Aquatic Toxicity Testing using the Nematode, Caenothabditits elegans," Environmental Tox. and Chem. 9: 1285-1290, 1990.
Stringham et al., "Temporal and Spatial Expression Patterns of the Small Heat Shock (hsp16) Genes in Transgenic Caenorhabditis elegans," Mol. Biol. Cell. 3: 221-233, 1992.
Stringham and Candido, "Transgenic hsp 16-lacZ Strains of the Soil Nematode Caenothabditis elegans as Biological Monitors of Environmental Stress," Environmental Toxicology and Chemistry 13(8): 1211-1220, 1994.
Stringham and Candido, "Targeted Single-Cell Induction of Gene Products in Caenorhabditis elegans: A New Tool for Developmental Studies," Journal of Experimental Zoology 266: 227-233, 1993.
Sanders, B., "Stress Proteins in Aquatic Organisms: An Environmental Perspective," Critical Reviews in Toxicology 23(1): 49-75, 1993.
Guven et al., "Evaluation of a stress-inducible transgenic nematode strain for rapid aquatic toxicity testing," Aquatic Toxicology 29: 119-137, 1994.
Fire et al., "A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans," Gene 93: 189-198, 1990.
Fire, A., "Integrative transformation of Caenorhabditis elegans," EMBO Journal 5(10): 2673-2680, 1986.
Candido Edward Peter Mario
Jones Donald
Stringham Eve Gabrielle
Campbell Bruce R.
University of British Columbia
LandOfFree
Biological systems incorporating stress-inducible genes and repo does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Biological systems incorporating stress-inducible genes and repo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Biological systems incorporating stress-inducible genes and repo will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-424658