Optics: measuring and testing – With sample preparation
Patent
1986-03-20
1988-05-31
McGraw, Vincent P.
Optics: measuring and testing
With sample preparation
356336, 356341, 377 11, 377 12, G01N 110, G01N 33487, G01N 1512, G01N 1514
Patent
active
047476850
ABSTRACT:
A biological microparticle inspection apparatus has a reactor in which blood containing T cells is mixed with latex-labeled antibodies and T cells are coupled with the antibodies to form complexes. A particle size measurement unit is provided for measuring the size of the particles contained in the blood, including the complexes. A signal processing unit processes the measured sizes of the particles outputting signals only when the measured sizes fall within a range of the size of the complex, and counts the number of the output signals. The number of the output signals corresponds to the number of the complexes and hence the T cells.
REFERENCES:
patent: 3977995 (1976-08-01), Louderback et al.
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patent: 4174952 (1979-11-01), Cannell et al.
patent: 4348107 (1982-09-01), Leif
The Review of Scientific Instruments, vol. 43, No. 3, pp. 404-409, "Fluorescence Activated Cell Sorting"; W. A. Bonner et al., Mar. 1972.
The Review of Scientific Instruments, vol. 44, No. 9, pp. 1301-1310, "A New Multiparameter Separator for Microscopic Particles and Biological Cells"; J. A. Steinkamp et al., Sep. 1973.
Kabushiki Kaisha Toshiba
McGraw Vincent P.
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