Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1983-11-25
1986-05-27
Warden, Robert J.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435 31, 435287, 435296, 206306, 374157, 374178, C12M 134, C12M 100, C12M 124, C12Q 122
Patent
active
045915660
ABSTRACT:
A system is disclosed for monitoring sterilization of biologically contaminated material from hospitals and the like. An autoclave sterilization unit heats biologically contaminated materials with steam for a time sufficient to kill the contaminants therein. A biological indicator probe provides for inserting a test vial into the central portion of the materials being treated. After sterilization, the probe is removed and the test vial is cultured to determine whether the spores of the Bacillus stearothermophilus, or the like (e.g. Bacillus sp.) present are still viable. If the test vial has been completely inactivated, it is an indication that decontamination of the other materials in the autoclave is complete. The test probe comprises a rod of heat resistant material having an end chamber closable by a rotatable sleeve. In a position aligning window openings in the chamber and in the sleeve, the test vial may be inserted. Rotation of the sleeve locks the test vial in place. The handle portion of the probe may have a laterally extending recess containing a thermocouple with wires extending out through the rod via a plug or connector insert for connection to an external temperature gauge for directly monitoring temperature of the material being sterilized while the test vial is being exposed to the high temperature steam. The probe can also be used in ethylene oxide sterilization and the like.
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Bio-Environmental Systems, Inc.
Deck Randall E.
Mosely Neal J.
Warden Robert J.
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