Biological indicator for sterilization processes

Details Biological indicator for sterilization processes Biological indicator for sterilization processes

1986-12-24

1988-05-10

Focarino, Margaret A.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435 31, 435289, 435810, C12M 124

Patent

active

047435372

ABSTRACT:
A sterility indicator which comprises means defining an elongated outer compartment having liquid impermeable and substantially non-gas absorptive walls, said compartment having a closed end and a first opening at a distal end thereof and a second opening which is gas transmissive and bacteria-impermeable disposed at least partially around the perimeter of said outer compartment and located near said first opening, with a predetermined number of viable microorganisms suitable for monitoring steam sterilization being confined within said outer compartment adjacent said second opening, and a predetermined number of viable microorganisms suitable for monitoring gas sterilization being confined within said outer compartment away from said second opening and opposite said distal end; a closure member normally closing said first opening and movable to a second position to also close said second opening; and an inner compartment containing a nutrient medium disposed within said outer compartment with said nutrient medium being out of direct contact with said microorganisms, said inner compartment being adapted to open in response to external pressure thereby permitting said nutrient medium to contact said microorganisms.

REFERENCES:
patent: 3346464 (1967-10-01), Ernst
patent: 3661717 (1972-05-01), Nelson
patent: 3752743 (1973-08-01), Henshilwood
patent: 4291122 (1981-09-01), Orelski
patent: 4304869 (1981-12-01), Dyke
patent: 4345028 (1982-08-01), Nelson et al.
patent: 4461837 (1984-07-01), Karle et al.
patent: 4528268 (1986-07-01), Anderson et al.
patent: 4579823 (1986-04-01), Ryder
patent: 4596773 (1986-06-01), Wheeler

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