Biological indicator for sterilization processes

Details Biological indicator for sterilization processes Biological indicator for sterilization processes

1980-08-14

1981-09-22

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435296, C12Q 122

Patent

active

042911222

ABSTRACT:
A biochemical test system is disclosed for use in a biological fermentation sterilization indicator. The carbohydrate component of a nutrient broth media is removed from the media. The carbohydrate component may be disposed in the cavity of an encapsulated sealed glass ampul which contains a non-carbohydrated test solution. Microorganism spores are also disposed in the cavity. Upon completion of sterilization the ampul is broken; the contents mix. Fermentation of viable spores yields growth and color change and is a positive test (unsatisfactory sterilization); no fermentation yields no growth, no color change and satisfactory sterilization. The separation of carbohydrate prevents non-microorganism fermentation in the test reaction, and the appearance of a "false positive".

REFERENCES:
patent: 3638918 (1972-02-01), Denholtz
patent: 3661717 (1972-05-01), Nelson
patent: 3791930 (1974-02-01), Saxholm

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