Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1981-02-23
1983-03-15
Hruskoci, Peter A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 7, 435 23, 435 24, 435 29, 435184, 435212, 435219, 435226, 436502, C12Q 100, C12Q 136, C12Q 138
Patent
active
043768199
ABSTRACT:
A p-nitrophenyl hydrolase contaminant present in Limulus coagulase sources interferes in, i.e., inhibits, coagulase-based assays for endotoxin. The contaminant inhibitor enzyme may be removed from coagulase by physical separation or by selective denaturation, for example by adjusting the pH of a coagulase solution to about from 8.5 to 11. Inhibitor-free coagulase is a novel composition. The coagulase activity of the composition is adjusted to a predetermined level to provide a standardized coagulase reagent for use in endotoxin assays.
REFERENCES:
patent: 3915805 (1975-10-01), Levin
patent: 3944391 (1976-03-01), Harris et al.
patent: 3954663 (1976-05-01), Yamamoto et al.
patent: 4024285 (1977-05-01), Beuk et al.
patent: 4038029 (1977-07-01), Teller et al.
patent: 4038147 (1977-07-01), Reno
patent: 4096091 (1978-06-01), Hopkins
patent: 4104030 (1978-08-01), Hopkins et al.
patent: 4107077 (1978-08-01), Sullivan et al.
patent: 4221865 (1980-09-01), Dubczak et al.
Solum, Thrombosis Research, 2, 1973, pp. 55-70.
Gaffin, Biorheology, 13, 1976, pp. 273-280.
Nachum et al., J. Invertebrate Pathology, 32, 1978, pp. 51-58.
Levin et al., J. Lab. Clin. Med., 75 (6), 1976, pp. 903-911.
Wildfeuer et al., Appl. Micro., 28 (5), 1974, pp. 867-871.
"Limulus Amebocyte Lysate for in Process Endotoxin Detection", Mallinckrodt, Inc., 12/72, pp. 1-3.
Mills, "Limulus Amebocyte Lysate for Detection of Endotoxins", Mallinckrodt Inc., 1978, pp. 1-32.
Reinhold et al., "A Technique for Quantitative Measurement of Endotoxin in Human Plasma", Soc. for Exp. Bio. Med., May 1971, p. 334.
Sullivan et al., Appl. Micro., 28 (6), 1974, pp. 1023-1026.
Hochstein et al., Bulletin of the Parental Drug Assoc., 27 (3), 1973, pp. 139-148.
Levin, New England J. Med., 288 (24), 1973, pp. 1297-1298.
Brown R. Clark
Cotter Richard
Young Susan K.
Baxter Travenol Laboratories Inc.
Flattery Paul C.
Flynn Lawrence W.
Hensley Max D.
Hruskoci Peter A.
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