Biological detection process using polymer-coated electrodes

Details Biological detection process using polymer-coated electrodes Biological detection process using polymer-coated electrodes

1983-08-15

1985-05-14

Williams, Howard S.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 25, 435288, 435291, 435817, 204403, 204418, 204 1T, 436 95, C12Q 154

Patent

active

045172919

ABSTRACT:
A method is described for the amperometric determination of the concentration in a biological sample of a constituent which is a substrate for an oxidase enzyme by (1) catalyzing the substrate with the enzyme, thereby consuming O.sub.2 and producing H.sub.2 O.sub.2 and (2) measuring the O.sub.2 consumption or H.sub.2 O.sub.2 production by means of an inert metal indicator electrode intimately coated with a thin film of a perfluorosulfonic acid polymer.
A reaction chamber is described which provides means for contacting the sample with the enzyme and means for making amperometric measurements utilizing inert metal indicator electrodes coated with a thin film of a perfluorosulfonic acid polymer.

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Inman et al, "the Immobilization of Enzymes on Nylon Structures and Their Use in Automated Analysis," Electrochem J. (1972) 129, 255-262.

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