Biological control of crown gall disease

Details Biological control of crown gall disease Biological control of crown gall disease

2006-11-28

2006-11-28

Fox, David T. (Department: 1638)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

C435S252200, C435S252300, C424S093200, C424S093400

Reexamination Certificate

active

07141395

ABSTRACT:
The present invention provides a method for controlling crown gall disease in plants using an effective quantity of α-proteobacteria that produces trifolitoxin (TFX). The present invention also provides a biocontrol agent for use in the above method, and a plant coated with the biological control agent. The biocontrol agent is characterized as a biologically pure culture of an α-proteobacteria strain that produces TFX, or an α-proteobacteria strain genetically engineered to produce TFX. The α-proteobacteria strain employed may include any one of the many strains ofAgrobacteriumcapable of producing crown galls, includingAgrobacterium vitisand, in particular,A. vitisF2/5. The α-proteobacteria strain employed may be genetically engineered to produce TFX by introducing a genetic construct into theAgrobacteriumso as to cause theAgrobacteriumto carry and express the tfx operon fromRhizobium. The bacteria may also be genetically engineered to produce TFX by introducing a pT2TFXK plasmid into theAgrobacterium. The biocontrol agent may also be the strainAgrobacterium vitisF2/5 (pT2TFXK), ATCC Patent Deposit Designation PTA-2356.

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