Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-05-25
1997-12-30
Guzo, David
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
9352523, 9353201, C12P 2100, C12N 121, C12N 1563
Patent
active
057029164
ABSTRACT:
A replicon, in which a nucleotide sequence encoding a cell killing function is regulatably expressed when the replicon is harbored in one type of host cell (primary host cell), so that cells harboring the replicon are killed under conditions under which the cell killing function is expressed, and the nucleotide sequence encoding the cell killing function is regulatably or constitutively expressed when the replicon is harbored in another type of host cell (secondary host cell), so that cells harboring the replicon are invariably killed or killed under conditions under which the cell killing function is expressed, may be used in a method of active biological containment of cells under defined environmental conditions. The biological containment principle may be utilized in the industrial production of a biosynthetic product by recombinant DNA techniques, when deliberately releasing a genetically engineered microorganism to the natural environment or in the preparation of a live vaccine. The expression of the cell killing function may be regulated by means of a promoter.
REFERENCES:
patent: 4436815 (1984-03-01), Hershbergor et al.
patent: 4634678 (1987-01-01), Solstrom et al.
patent: 4767708 (1988-08-01), Minkley et al.
Klemm, 1985, Fimbriae adhesions of Escherichia coli, Rev. Infect. Dis. 7, 321-339.
Molin, Soren; "Containment Scenarios" Manuscript (1990).
Contreras, et al,. Conditional-Suicide Containment System for Bacteria which Mineralize Aromatics, Applied & Environ. Microbiol., 57:1504 (1991).
Poulsen et al.; The gef Gene from E. coli is Regulated at the Level of Translation; Mol Microbiol, 5, 1639-1648 (1991).
Klemm, et al.; Fimbriae of Escherichia coli as carriers of heterologous antigenic sequences, Res. Microbiol., 141, 1013-1017 (1990).
Cuskey, S.M.; Use of the RK2 kila Gene for Biological Containment of Microoganisms Released to the Environment; To be submitted to: Applied & Env. Microbiol.; Cont. No. 657; Env. Res. Lab., U.S.E EPA.
"Fox, Contemplating Suicide Genes in the Environment", ASM News, 55:259 (1989).
Poulsen et al., A Family of Genes Encoding a Cell-Killing Function may be Conserved in all Gram-Negative Bacteria; Mol. Microbiol., 1989, 3, 1463-1472 (1989).
Molin, Soren, Untitled Manuscript Relating to Suicide Systems (ref. IX).
Knudsen and Karlstrom, "Development of Efficient Suicide Mechanisms for Biological Containment of Bacteria Applied & Environ. Microbiol.," 57:85-92 (1991).
"Bej. et al., Model Suicide Vector for Containment of Genetically Engineered Microorganisms", Applied & Environ. Microbiol., 54:2472 (1988).
Steffan et al.; Inducible Systems for the Containment of Genetically Improved Gram Negative Bacteria; EERO Workshop, Konigslutter, Germany, Apr. 28 -May 1, 1990.
Hill et al., in Drlica et al. (eds) The Bacterial Chromosome, American Society for Microbiology, Washington D.C., pp. 335-340 (1990).
Mahan et al. in Drlica et al. (eds) The Bacterial Chromosome, American Society for Microbiology, Washington D.C., pp. 341-349 (1990).
Smith, Mechanism and Control of Homologous Recombination in E. coli; Ann. Rev. Genet. 21:179-201 (1987).
Watt, et al., Homology Requirement for Recombination in E. coli; PNAS (USA), 82:4768-72 1985).
Mizuno, et al., A Unique Mechanism Regulatin Gene Experssion; Translational Inhibition by a Complementary RNA Transcript (micRNA); PNAS (USA), 81:1966-70 (1984).
Old and Primrose, Principles of Gene Manipulation, vol. 2, pp. 153-162 (Blackwell Scientific Publication 4th ed., 1989).
Jorgensen, et al.; In vivo Genetic Engineering: Homologous Recombination as a Tool for Plasmid Construction Gene, 96:37-41 (1990).
Agterberg, et al.; Use of Outer Membrane Protein PhoE as Carrier for the Transport of a Foreign antigenic Determinant to the Cell Surface of E. coli K-12; Gene, 59:145-150 (1987).
Wu, et al.; Expression of Immunogenic Epitopes of Hepatitis B Surface Antigen with Hybrid Flagellin Protein by a Vaccine Strain of Salmonella; PNAS (USA), 86:4726-30 (1989).
Izant and Weintraub; Constitutive and Conditional Suppression of Exogenous and Endogenous Genes by Anti-Sense RNA; Science, 229:345-352 (1985).
Travers; Regulation by Anti-Sense RNA; Nature, 311-:416 (1984).
R.H.A. Plasterk et al., Proc. Natl. Acad. Sci. USA 80, 1983, pp. 5355-5358; "DNA inversions in the chromosome of Escherichia coli and in bacteriopgage Mu: Relationship to other site-specific recombination systems."
G. Mertens et al,. EMBO J. 3, No. 10, 1984, pp.2415-2421; "Gin-mediated site-specific recombination in bacteriophage Mu DNA: overproduction of the protein and inversion in vitro."
J. Zieg and M.I. Simon, Proc. Natl. Acad. Sci. USA 77, No. 7, 1980, pp. 4196-4200; "Analysis of the nucleotide sequence of an invertible controlling element."
P. Klemm, EMBO J. 5, 1986, pp. 1389-1393; "Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli. "
Maniatis et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, 1982 (whole textbook) Attached are: pp. 280-281, p.388, p.256; (pages taken are from three different articles.)
Bagdasarian et al., Gene 16, 1981, pp. 237-247; "Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudononas. "
Lovett and Keggins, Meth. in Enzymol. 68, 1979, pp. 342-357; "Bacillus subtilis as a Host for Molecular Cloning."
Bech et al., The EMBO Journal 4, 1985, pp. 1059-1066; "Sequence of the relB transcription unit from Escherichia coli and identification of the relb gene."
Clark and Maaloe, J. Mol. Biol. 23, 1967, pp. 99-112; "DNA Replication and the Division Cycle in Escherichia coli. "
Clewell and Helinski, Proc. Natl. Acad. Sci. USA 62, 1969, pp. 1159-1166; "Supercoiled Circular DNA-Protein Complex in Escherichia coli: Purification and Induced Conversion to an Open Circular DNA Form."
Birnboim et al., Nucl. Acids Res. 7, 1979, pp. 1513-1523; "A rapid alkaline extraction procedure for screening recombinant plasmid DNA."
Stougaard and Molin, Anal. Biochem. 118, 1981, pp. 191-193; "Vertical Dye-Buoyant Density Gradients for Rapid Analysis and Preparation of Plasmid DNA."
R. Simon, Biotechnology, Nov. 1983, pp. 784-791; "A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria."
G. Skogam, J. Nilsson, P. Gustafsson, Gene 23, 1983, pp. 105-115; "The Use of a partition Locus to increase stability of tryptophan-operon-bearing plasmids in Escherichia coli. "
Yansura and Henner, Proc. Natl. Acad. Sci. 81, 1984, pp. 439-443 --(Identical With Ref. No. AL2 Below); "Use of the Escherichia coli lac repressor and operator to control gene expression inBacillus subtilis. "
Bonner, T.I. et al., J. Mol. Biol. 81, 1973, pp. 123-135; "Reduction in the Rate of DNA Reassociation by Sequence Divergence."
Beltz, G.A. et al., Meth Enzymol. 100, 1983, pp.266-285; "Isolation of Multigene Families and Determination of Homologies by Filter Hybridization Methods."
Gerdes et al., J. Bacteriol. 161, 1985, pp.292-298; "Stable Inheritance of Plasmid R1 Requires Two Different Loci."
Prentki et al., Gene 17, 1982, pp. 189-196; "A modified pBR322 vector with improved properties for the cloning, recovery, and sequencing of blunt-ended DNA fragments."
Miki et al., J. Bacteriol. 141(1), 1980, pp. 87-99.
Sharp et al., J. Mol. Biol. 75, 1973, pp. 235-255; "Electron Microscope Heteroduplex Studies of Sequence Relations among Plasmids of Escherichia coli. "
Ogura & Hirago, Proc. Natl. Acad. Sci. USA 80, 1983, pp. 4784-4788; "Mini-F plasmid genes that couple host cell division to plasmid proliferation."
Yanzura and Henner, Proc. Natl. Acad. Sci. USA 81, Jan. 1984, pp. 439-443 -- .star-solid..star-solid..star-solid.Identical With Ref. No. AD2 Above.star-solid..star-solid..star-solid..
Klemm et al., Mol. Gen. Genet. 199, 1985, pp.410-414; "The fim genes responsible for synthesis of type 1 fimbriae in Escherichia coli, cloning and genetic organization."
Gerdes et al., Proc. Natl. Acad. Sci. USA 83, 1986, pp. 3116-3120; "Unique type of plasmid maintenance functio
Andersson Poul Kirketerp
Gerdes Kenn Axo
Klemm Per
Molin S.o slashed.ren
Cooper Iver P.
Guzo David
GX Biosystems A/S
Schwartzman Robert
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