Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1993-03-08
1995-08-15
Lacey, David L.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530402, 536 2532, 536 266, G01N 3700, C07K 113, C07D21100
Patent
active
054420458
ABSTRACT:
Novel fluorescent dyes based on the rhodol structure are provided. The new reagents contain functional groups capable of forming a stable fluorescent product with functional groups typically found in biomolecules or polymers including amines, phenols, thiols, acids, aldehydes and ketones. Reactive groups in the rhodol dyes include activated esters, isothiocyanates, amines, hydrazines, halides, acids, azides, maleimides, aldehydes, alcohols, acrylamides and haloacetamides. The products are detected by their absorbance or fluorescence properties. The spectral properties of the fluorescent dyes are sufficiently similar in wavelengths and intensity to fluorescein or rhodamine derivatives as to permit use of the same equipment. The dyes, however, show less spectral sensitivity to pH in the physiological range than does fluorescein, have higher solubility in non-polar solvents and have improved photostability and quantum yields.
REFERENCES:
Whitaker et al., Anal. Biochem., 1992, 207:267.
Goding et al., Monoclonal Ab., Principles & Practice, pp. 241-248.
Haugland Richard P.
Whitaker James E.
Eisenschenk F. Christopher
Helfenstein Allegra J.
Lacey David L.
Molecular Probes, Inc.
Skaugset Anton E.
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