Biological assay for microbial contamination

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 4, 435 29, 435 34, 435810, C12Q 106

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active

055500323

ABSTRACT:
Methods and kits for detecting the presence of and quantifying the concentration of viable microorganisms in a liquid sample are provided. Enzymes present in viable microorgansims reduce an indicator reagent to produce a visible color change. No titrations or dilutions of the sample are necessary. Variations of the test are described which can be used to detect a threshold level of viable microorganisms, to detect the presence or absence of viable microorganisms and to quantify the concentration of viable microorganisms in a liquid sample. The results are obtained rapidly with those for the detection available within thirty minutes and those for the quantification available within thirteen hours.

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