Biofilm preparation using potassium permanganate

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving fixed or stabilized – nonliving microorganism,...

Reexamination Certificate

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C436S172000, C424S640000

Reexamination Certificate

active

07871791

ABSTRACT:
A method for preparing a biofilm includes the steps of rinsing the biofilm. There is the step of staining the biofilm with potassium permanganate and water.

REFERENCES:
patent: 6569615 (2003-05-01), Thatte et al.
patent: 2003/0079758 (2003-05-01), Siegel et al.
patent: 2005/0037451 (2005-02-01), Chen et al.
patent: 2006/0275847 (2006-12-01), Goodyear et al.
patent: WO 03/011347 (2003-02-01), None
EPA Guidance Manual: Alternative Disinfectants and Oxidants; Chapter 5, Apr. 1999, pp. 1-15 www.epa.gov/ogwdw/mdbp/pdf/alter/chapt—5.pdf ,downloaded Nov. 19, 2008.
Whitchurch, Cynthia B.; Tolker-Nielsen, Tim; Ragas, Paula C.; Mattick, John S., “Extracellular DNA Required for Bacterial Biofilm Formation,” www.sciencemag.org, Science, (vol. 295), (p. 1487), (Feb. 22, 2002).
Koerstgens V. et al., “Influence of Calcium Ions on the Mechanical Properties of a Model Biofilm of MucoidPseudomonas aeruginosa,” Water Science and Technology, vol. 43 ( No. 6), pp. 49-57, (2001).

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