Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1995-05-01
1997-01-28
Kight, John
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 4, 435 29, 435 731, 4352541, 4352551, 435 47, 435 49, C12Q 126, C12Q 100, C12Q 102, C12P 3500
Patent
active
055977049
ABSTRACT:
A method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid. The method including obtaining a cell preparation from a microorganism which produces D-amino acid oxidase, the preparation being a cell-free extract or a suspension of permeated cells; adding a D-.alpha.-amino acid to the preparation; after the adding step, heating the preparation at 50.degree.-75.degree. C. for 5-60 minutes; and incubating cephalosporin C in the preparation. Also disclosed are a method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid in the absence of exogenous H.sub.2 O.sub.2, and a method of screening for a D-amino acid oxidase-producing microorganism.
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Chu Wen-Shen
Hsu Wen-Hwei
Lee Yun-Huey
Food Industry Research and Development Institute
Kight John
Leary Louise N.
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