Biochemical sensor system with increased sensitivity by...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S006120

Reexamination Certificate

active

06872810

ABSTRACT:
The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater then 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method o end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labelled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labelling can be accomplished by providing a nucleic acid, providing labelled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.

REFERENCES:
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patent: 5795738 (1998-08-01), Grandi et al.
patent: 5858802 (1999-01-01), Chai-Gao et al.
patent: 6238866 (2001-05-01), Yeh et al.
patent: 6261779 (2001-07-01), Barbera-Guillem et al.
patent: 6406894 (2002-06-01), Hoersch et al.
patent: WO 9727317 (1997-07-01), None

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