Stock material or miscellaneous articles – Hollow or container type article – Polymer or resin containing
Patent
1988-06-23
1991-05-07
Seidleck, James J.
Stock material or miscellaneous articles
Hollow or container type article
Polymer or resin containing
422102, 428 3692, G01N 33545, B01L 300
Patent
active
050135902
DESCRIPTION:
BRIEF SUMMARY
The invention relates to a reaction vessel made of a chemically inert thermoplastic with a substance incorporated therein, the substance having molecules at the surface of the receptacle with reactive groups of atoms.
BACKGROUND OF THE INVENTION
Reaction receptacles are used in biological laboratories or in biochemical processing and especially in clinical laboratory diagnosis. They serve to carry out biochemical reactions of the most diverse kinds, employing chemical reactions with biospecific compounds where at least one of the reagents is a protein, namely a protein molecule or a fragment of it. Such analysis is carried out large-scale on blood samples, for instances to test patients for pathological symptoms or to analyze donor blood in blood banks. The chemical reactions that are carried out are most diverse in nature, and thus they include enzyme reactions, hormone reactions, antigenantibody reactions or blood-coagulating reactions, the last usually taking place at the beginning of a blood test.
Such reaction receptacles are used today in large numbers at low prices so as to be economically justifiable for instance in clinical large-scale labs of conventional size.
Accordingly the material used for these reaction receptacles has been a thermoplastic which allows making economical reaction receptacles and which, on account of its chemical inertness, does not affect the reactions taking place in the receptacle.
In the state of the art, the reagent required for the biochemical reaction must be added to the test material in such reaction receptacles. This entails a disadvantageous additional operational step. Moreover another drawback must frequently be incurred, namely that the added reagent must be separated again before the next test is carried out since otherwise there will be interference and, again, additional operational steps must be carried out.
SUMMARY OF THE INVENTION
Accordingly it is the object of the present invention to create a reaction receptacle wherein biochemical reactions can be carried out in less time and with less labor.
Briefly described, the invention includes a reaction receptacle made of a chemically inert thermoplastic to carry out chemical reactions with biospecific compounds, preferably for use in medical laboratory diagnosis. The plastic is so treated by the addition of a non-migrating incorporated substance prior to its thermoplastic shaping that, following this shaping, molecules with reactive groups of atoms of the incorporated substance are present at least at the inside surface of the receptacle.
In the invention, a substance is added to the plastic of the reaction receptacle so it will be incorporated into this plastic, when this plastic melts but before the plastic is thermoplastically shaped so that the substance be non-migrating. "Non-migrating"means with respect to the present technology that the added substance shall be so bound to the plastic that it will not be transferred, not even in slight amounts, to the test sample. The added substance comprises molecules with chemically reactive groups of atoms. The incorporation into the plastic is carried out in such a way that at least a substantial number of the reactive groups of atoms of the added molecule of said substance shall be located and exposed at the bare surface of the plastic. Accordingly, the reaction receptacle of the invention evinces reactive groups of atoms on at least its inside surface which come in contact with the filled test sample, where these reactive groups of atoms can be directly used for the desired reaction without separating from the plastic, or else allow binding a desired ligand which, together with the test sample, will result in the desired reaction. The significant advantage obtained thereby is that the molecule of the substance bearing the reactive groups of atoms or the ligands bound to that molecule are available for the reaction but do not enter the test sample.
After the reaction has been carried out, the test sample can be removed without being contaminated by the molec
REFERENCES:
Chemical Abstracts, Textiles, vol. 79, p. 71, 1973.
Nerbe Jurgen F.
Wonne Karl-Georg
Farley Walter C.
Seidleck James J.
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