Biochemical assay plate and method for making the same

Details Biochemical assay plate and method for making the same Biochemical assay plate and method for making the same

1994-11-14

1997-10-14

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

432 55, 432 57, 4352871, 4352872, 4352879, 4352883, 435990, 436524, 436528, 436531, 436532, 436164, 436809, 436800, G01N 33543

Patent

active

056771975

ABSTRACT:
An assay system for detecting the binding of a mobile reactant to an immobilized reactant on an assay plate. The assay plate includes a substrate having an assay spot deposited thereon. The assay spot includes the immobilized reactant. In the present invention, a carrier dye is included in the assay spot, the amount of the carrier dye indicating the amount of the immobilized reactant that is present in the assay spot. In the preferred embodiment of the present invention, the assay spot is generated by depositing liquid on the assay plate at a location corresponding to the assay spot. The carrier dye is dissolved in the liquid and remains after the liquid evaporates. The amount of carrier dye in each spot may be measured spectroscopically and provides a means for identifying defective assay plates. The amount of carrier dye in each spot may also be recorded on the assay plate and used in conjunction with measurements of the amount of material bound to the assay spot to determine the concentration of a reactant in a solution being tested for the reactant.

REFERENCES:
patent: 4748042 (1988-05-01), Linnecke et al.
patent: 5073340 (1991-12-01), Covington et al.

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