Biochemical analysis of antioxidant function of lymphocytes in c

Details Biochemical analysis of antioxidant function of lymphocytes in c Biochemical analysis of antioxidant function of lymphocytes in c

1996-06-19

1999-11-16

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Culture medium, per se

435 4, 435 14, 435 29, 435372, 435375, 435387, 435405, 435406, 435 2, C12N 500, A01N 102, C12Q 102, G01N 100

Patent

active

059856653

ABSTRACT:
The present invention provides a cell culture medium useful for a biochemical analysis of antioxidant function in human lymphocytes, said medium comprising, a buffered, serum-free solution containing the following ingredients: a carbohydrate selected from the group consisting of glucose and a compound biologically capable of producing glucose in the cells, a biologically usable form of pantothenic acid, choline or a biological usable form of a substance capable of producing choline in the cells, inorganic ions comprising chloride, phosphate, calcium, magnesium, potassium, sodium, and iron in a biologically utilizable form, cumene hydroperoxide, deionized water, and a mitogen in an amount effective to stimulate the lymphocytes being assayed; said buffered, serum-free solution having a pH from about 6.8 to 7.6, said cell culture medium characterized by being effective to determine nutritional deficiencies, inadequacies, and imbalances and to biochemically analyze antioxidant function of the lymphocytes. Also provided is a method of biochemically analyzing cellular antioxidant function in an individual comprising the steps of: inoculating the cell culture medium of the present invention with lymphocytes from said individual; incubating the inoculated cell culture medium; and comparing the response of the lymphocytes with an average response of lymphocytes from a control group of individuals.

REFERENCES:
patent: 4499064 (1985-02-01), Shive
patent: 4927762 (1990-05-01), Darfler

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