Biocatalyst immobilization with a reversibly swelling and shrink

Details Biocatalyst immobilization with a reversibly swelling and shrink Biocatalyst immobilization with a reversibly swelling and shrink

1989-09-05

1990-12-04

Naff, David M.

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

435177, 436528, 436531, 530812, 530817, C12N 1104, C12N 1102, G01N 33545, G01N 33544

Patent

active

049753754

ABSTRACT:
A biocatalyst such as an enzyme or microbe is immobilized in a polymer gel having a phase transition temperature such that it is capable of reversibly swelling and shrinking by a change in temperature. By lowering the temperature, the polymer gel is caused to swell and a biocatalyst is absorbed therein and by raising the temperature, the polymer gel is caused to shrink and immobilize the biocatalyst. The biocatalyst may then be released by cooling the polymer to cause it to swell. Only a portion of the polymer gel may be subjected to temperature change to immobilize the biocatalyst only in a desired portion.

REFERENCES:
patent: 3421892 (1969-01-01), Taylor
patent: 3421893 (1969-01-01), Taylor
patent: 3705084 (1972-12-01), Reynolds
patent: 4070348 (1978-01-01), Kraemer et al.
patent: 4364385 (1982-12-01), Lossef
Dahlqvist et al., "Hydrolysis of B-Galactosides Using Polymer-Entrapped Lactase-Free Milk", Biotechnology and Bioengineering XV, pp. 395-402 (1973).
Mosbach, "Matrix-Bound Enzymes", Acta Chem. Scand., 24 pp. 2084-2100 (1970).

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