Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1988-04-25
1991-02-26
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435177, 435178, 435179, 435180, C12P 706, C12N 1110, C12N 1112, C12N 1108
Patent
active
049961500
ABSTRACT:
An immobilized biocatalyst suitable for fermenting to produce ethanol is prepared by mixing a biocatalyst such as a microorganism with a reaction product of a homogeneous dispersion of an anionic polysaccharide such as alginate and a cationic polymer such as polyethyleneimine, and combining the resultant dispersion with an oil phase to form beads. A surtactant may be present when combining the dispersion with the oil phase. A water soluble-oil insoluble curing powder includinga salt of a multivalent cation such as calcium chloride is mixed with the beads in the oil phase to gell and dehydrate the beads and to prevent the beads from adhering to one another until individual bead surfaces become hardened.
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Mohamed et al., Biotechnology Letters, vol. 4, No. 9, 1982, pp. 611-614.
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Chemical Abstracts, vol. 95, No. 15, Oct. 12, 1981, p. 513, Abstract No. 130963p, Columbus, Ohio, U.S.; S. Birnbaum et al: "Covalent stabilization of alginate gel for the entrapment of living whole cells", & Biotechnol. Lett. 1981, 3(8), 393-400.
Immobilization of Growing Cells by Polyethyleneimine-Modified Alginate; John J. Joung, Cavit Akin, and G. P. Royer; Applied Biochemistry and Biotechnology; vol. 14, 1987.
A Horizontal Packed-Bed Bioreactor to Reduce CO.sub.2 Gas Holdup in the Continuous Production of Ethanol by Immobilized Yeast Cells, Takeshi Shiotani and Tsuneo Yamane; European Journal of Applied Microbiology and Biotechnology (1981) 13: 96-101.
Akin Cavit
Joung John J.
Royer Garfield P.
Amoco Corporation
Galloway Norville B.
Magidson William H.
Mehurst Ralph C.
Naff David M.
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