Biocatalyst entrapped in a silk fibroin membrane

Details Biocatalyst entrapped in a silk fibroin membrane Biocatalyst entrapped in a silk fibroin membrane

1988-04-01

1991-03-12

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

204403, 435182, 435817, C12N 1102, C12N 1104, C12Q 100, G01N 2726

Patent

active

049992953

ABSTRACT:
A silk fibroin membrane containing an entrapped biocatalyst such as an enzyme or microorganism is provided by forming a mixture of a biocatalyst solution and a silk fibroin solution, forming a membrane by casting the mixture, drying the membrane and mechanically treating the resultant membrane at a temperature and humidity to produce the membrane in .beta.-form and to structurally stabilize the membrane. Mechanically treating may be by stretching or compressing. A biocatalyst sensor is formed by coating an electrically conductive substrate with a gas-permeable layer and coating the gas-permeable layer with the silk fibroin membrane containing an entrapped biocatalyst.

REFERENCES:
Demura et al., "Structural Characteristics of Silk and Application to Enzyme-Fixed Materials," report published in the 57th Lecture Meeting of the Japanese Society of Sericultural Science at Yatabe-cho, Ibaraki-ken, Japan on Apr. 3, 1987.
Demura et al., "Fine Structure of Silk Fibroin Membrane and Activity of Immobilized Enzyme," report published in the 36th Convention of the Society of Polymer Science, Japan at Kokuritsu Kyoto Kokusai Kaikan on May 29, 1987.
Kobos, R. K., Trends in Analytical Chemistry, vol. 2, No. 7, 1983, pp. 154-157.
Kuzuhara et al., Journal of Biotechnology, vol. 5, 1987, pp. 199-207.

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