Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-11-26
1998-11-03
Hendricks, Keith D.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
4353201, 435 26, 435190, 435207, 4352523, 43525233, 536 232, 536 234, G01N 3353, C12Q 126, C12N 1570, C12N 904
Patent
active
058306730
ABSTRACT:
The invention relates to a plasmid carrying a selenium-specifying DNA seqce of the E. coli fdhF gene upstream of the E. coli lac'Z gene which permits the incorporation of selenocystein into .beta.-galactosidase. Particular plasmids according to the invention are pRM2, deposited at the ATCC under No. 75594 and pRM4, deposited at the ATCC under No. 75595. The invention also relates to microorganisms transformed with a plasmid according to the invention having selenium dependent .beta.-galactosidase activity. The invention provides a method for the quantitative determine of selenium in selenium derivatives in a biological sample comprising incubating microorganisms according to the invention in a suitable medium also containing said sample and measuring the level of .beta.-galactosidase activity. The biological sample may be, e.g. a blood sample or a food sample.
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Engelberg-Kulka Hanna
Reches Myriam
Hendricks Keith D.
Yissum Research Development Company Ltd. of Hebrew Univ. of Jeru
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