Bioassay of parathyroid hormone

Details Bioassay of parathyroid hormone Bioassay of parathyroid hormone

1983-03-21

1985-04-02

Nucker, Christine M.

Chemistry: analytical and immunological testing

Thyroid hormone tests

422 61, 436503, 436504, 436804, 436808, 436817, 436824, 436825, G01N 3356, G01N 3358, G01N 3360

Patent

active

045088284

ABSTRACT:
A method is disclosed for extraction, concentration, and bioassay measurement of the concentration of bioactive parathyroid peptides in biological or other fluids. Parathyroid peptides are extracted from the fluid by a support-antibody matrix specific to the N-terminal region. The bioassay is directed to the extracted parathyroid peptides. The volume of the medium containing the extracted parathyroid peptides and tissue, cells or tissue extracts with adenylate cyclase-coupled parathyroid hormone receptors may be chosen to be significantly less than the volume of biological or other fluid being assayed.

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Teitelbaum et al., Endocrinology 111: 1524-1533, (1982).
Nissenson et al., Advances in Experimental Medicine and Biology 151: 549-559, (1982).
Avioli et al., Abstracts: Mineral Metabolism; Pharmaceutical Hormone, Calcified Tissue Int'l 33: 318, (1981).

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