Bioassay for toxic substances activated by metabolic enzyme syst

Details Bioassay for toxic substances activated by metabolic enzyme syst Bioassay for toxic substances activated by metabolic enzyme syst

1995-11-01

1998-12-01

Knight, John

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435975, C12Q 126

Patent

active

058436960

ABSTRACT:
A method for bioassaying for metabolic activation of toxicants from a xenobiotic compound by a metabolic enzyme system includes incubating the xenobiotic compound with a metabolic enzyme system known to produce toxicants during normal metabolic degradation processes and with a mitochondrial membrane preparation competent for enzymatic electron transfer. The production of a toxicant has a detrimental effect upon the electron transfer activity of the mitochondrial membrane preparation which can readily be assayed by observing changes in concentration of a selected redox indicator.

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