Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1985-12-27
1989-02-28
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 15, 435 18, 435 25, 435 26, 435810, C12Q 100, C12Q 148, C12Q 134, C12Q 126, C12Q 132
Patent
active
048085170
ABSTRACT:
An assay method is disclosed to test for the presence of toxic substances in environmental samples. The assay uses a suspension of mitochondrial membranes carrying enzymes which can be driven by reverse electron transfer to reduce NAD+ to NADH. The competency of the mitochondrial membrane to accomplish this reduction in the presence of the environmental sample is measured spectrophotometrically to test for toxicants.
REFERENCES:
Blondin et al., Archives Biochem. Biophys., 132:509-523 (1969).
Jolly et al., Archives Biochem. Biophys., 130: 191-211 (1969).
Crane et al., Biochem. Biophys. Acta, 22: 475-487 (1956).
Hatefi et al., Biochem. Biophys. Acta, 27: 83-88 (1958).
Hansen et al., Biochem. Biophys. Acta, 81: 214-222 (1964).
Lenag et al., J. Biol. Chem., 241: 5260-5265 (1966).
Beckman Instruments, "Microtox Model 2055 Toxicity Analyzer System."
Bitton, "Bacterial and Biochemical Tests for Assessing Chemical Toxicity in the Aquatic Environment: A Review," Critical Reviews in Environ. Control, 13:1, pp. 51-52 (1984).
Ogata et al., "Classification of Potentially Toxic Chemicals based on Their Effects on Mitochondrial Respiration," Phys. Chem. and Phys. and Med. NMR, 15, pp. 229-232 (1983).
Blondin George A.
Harkin John M.
Kepplinger Esther M.
Wisconsin Alumni Research Foundation
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