Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Rodent cell – per se
Reexamination Certificate
2006-01-10
2006-01-10
Nolan, Patrick J. (Department: 1644)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Rodent cell, per se
C435S325000
Reexamination Certificate
active
06984520
ABSTRACT:
An assay method for TSH-R autoantibodies or TSH comprises contacting a test sample, in the presence or absence of TSH, with cells from a clone expressing human TSH-R transfected with a reporter construct comprising cDNA of both (i) a reactant, such as an enzyme, capable of causing a measurable response when brought into contact with a corresponding substrate, such as a protein, and (ii) a promoter containing cyclic AMP (cAMP) response elements (CREs), whereby cAMP levels vary with expression of the reactant. Also disclosed are related kits, reporter constructs and related biological material.
REFERENCES:
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Himmler et al., “Functional Testing of Human Dopamine D1and D5Receptors Expressed in Stable cAMP-Responsive Luciferase Reporter Cell Lines”,Journal of Receiptor Research,vol. 13, No. 1/04, Jan. 1993, pp. 79-94.
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L. Persani et al., “Measurement of cAMP accumulation in Chinese hamster ovary cells transfected with the recombinant human TSH receptor (CHO-R): a new bioassay for human thyrotropin”,J. Endocrinol. Invest.,vol. 16, Jul. 1993, pp. 511-519.
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P.F. Watson et al., “A new chemiluminescent assay for the rapid detection of thyroid stimulating antibodies in Graves' disease”,Clin. Endocrinology,vol. 49, Nov. 1998, pp. 577-581.
Nolan Patrick J.
University of Wales College of Medicine
Young & Thompson
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