Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1991-12-10
1994-03-29
Hill, Jr., Robert J.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
435 691, 4351723, 4352523, 4353201, C12N 1512, G01N 3000
Patent
active
052984290
ABSTRACT:
Bioassays are disclosed which are useful for determining whether a compound is a hormone receptor agonist (i.e., is capable of promoting the transcription-activation activities of such receptors) or a hormone receptor antagonist (i.e., is capable of blocking the transcription-activation activities of such receptors). The invention bioassay is conducted by culturing test cells in the presence of at least one compound whose ability to function as a ligand for said receptor protein (or functional engineered or modified forms thereof) is sought to be determined. Alternatively, test cells are cultured in medium containing increasing concentrations of at least one compound whose ability to inhibit the transcription activation activity of hormone receptor agonists is sought to be determined, and a fixed concentration of at least one agonist for the receptor protein. Test cells employed in the practice of the present invention contain non-endogenous DNA which expresses hormone receptor (or functional modified forms thereof) and a DNA sequence encoding a hormone response element operatively linked to a reporter gene. The cultured cells are monitored for evidence of transcription of the reporter gene as a function of the concentration of test compound in the culture medium. The variation in transcription levels of the reporter gene as a function of concentration of test compound indicates the ability of test compound to promote or inhibit activation of transcription.
REFERENCES:
Nature 305: 551-554, 6 Oct. 1983, Dean et al. A 5'-Flanking Sequence Essential for Progesterone Regulation of an Ovalbumin Fusion Gene.
J. Biol. Chem. 258: 12693-12701, 25 Oct. 1983, Lai et al., Regulated Expression of the Chicken Ovalbumin Gene in a Human Estrogen-responsive Cell Line.
Nature 312: 779-781, 27 Dec. 1984, Miesfeld et al., Characterization of a Steroid Hormone Receptor Gene and mRNA in Wild-Type and Mutant Cells.
P.N.A.S. 82: 7889-7893, Dec. 1985, Walter et al., Cloning of the Human Estrogen Receptor cDNA.
Mol. & Cell. Biol. 2: 1044-1051, Sep. 1982, Gorman et al., Recombinant Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells.
Nature 308: 513-519, 5 Apr. 1984, Karin et al., Characterization of DNA Sequences Through Which Cadmium and Glucocorticoid Hormones Induce . . . .
Cell 46: 389-399, Aug. 1986, Miesfeld et al., Genetic Complementation of a Glucocorticoid Receptor Deficiency by Expression of Cloned Receptor cDNA.
Evans Ronald M.
Giguere Vincent
Hollenberg Stanley M.
Hill Jr. Robert J.
The Salk Institute for Biological Studies
Ulm John D.
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