Bioactive chip mass spectrometry

Details Bioactive chip mass spectrometry Bioactive chip mass spectrometry

2005-05-03

2005-05-03

Warden, Jill (Department: 1743)

Chemistry: analytical and immunological testing

Nuclear magnetic resonance, electron spin resonance or other...

C436S174000, C436S175000, C436S177000, C436S063000, C436S501000, C436S518000, C422S050000, C422S051000, C422S051000, C422S068100, C435S006120, C435S007100

Reexamination Certificate

active

06887713

ABSTRACT:
The present invention is directed to a bioactive probe or chip (BC) that allows for the isolation of analytes, such as biomolecules, followed by modification or bioreaction on these said analytes. More specifically, the present invention relates to various methods and apparatuses that include the BC and further include characterization and identification technologies, such as Bioactive Chip Mass Spectrometry (BCMS). Within the context of the present invention, BC provides a method and device for the capture and subsequent modifying, such as digestion or derivatization, of an analyte. Further, real-time information regarding a variety of molecular interactions may be provided by techniques such as interaction analysis (IA). Finally, the variety of molecules are localized and concentrated thereby aiding in the identification and/or quantification of the molecules by techniques such as mass spectroscopy. In one embodiment, a method for performing the modification, or bioreaction, of biomolecules is disclosed. Preferably, the method involves capturing an analyte present within a sample by an interactive surface layer located in a separation site; washing unwanted portions of the sample from the surroundings of the captured analyte; transferring the captured analyte from the separation site to a modifying site; modifying or bioreacting the analyte to create a modified or bioreacted analyte. The modified analyte may then be subsequently characterized and/or identified by techniques such as mass spectrometry.

REFERENCES:
patent: 5304487 (1994-04-01), Wilding et al.
patent: 5632957 (1997-05-01), Heller et al.
patent: 5770272 (1998-06-01), Biemann et al.
patent: 5856082 (1999-01-01), Aebersold et al.
patent: 5872010 (1999-02-01), Karger et al.
patent: 5955729 (1999-09-01), Nelson et al.
patent: 6004770 (1999-12-01), Nelson
patent: 6225047 (2001-05-01), Hutchens et al.
patent: 6274089 (2001-08-01), Chow et al.
patent: 6322970 (2001-11-01), Little et al.
WO 94/28418 (PCT//US94/06064) (Hutchens et al).*
Karas, M., U. Bahr, A. Ingendoh, E. Nordhoff, B. Stahl, K. Strupat and F. Hillenkamp, Principles and application of matrix-assisted U- laser desorption/ionization mass spectrometry, Analytica Chimica Acta, 241 (1990) 175-185, Elsevier Science Publishers B. V., Amsterdam.
Amankwa, Lawrence N. and Werner G. Kuhr, Trypsin-Modified Fused-Silica Capillary Microreactor for Peptide Mapping by Capillary Zone Electrophoresis, Anal. Chem. 1992, 64, 1610-1613.

Affiliated with

Also associated with

Rating

Bioactive chip mass spectrometry does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Bioactive chip mass spectrometry, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bioactive chip mass spectrometry will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3434633