Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...
Patent
1989-11-07
1992-12-29
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
435178, 43524022, 43524023, 43524026, 4352404, 4352521, C12P 100, C12N 1110, C12N 500, C12N 112
Patent
active
051750937
ABSTRACT:
Bioactive cells are immobilized by suspending bioactive cells in an aqueous solution of a salt-free osmolarity adjuster, alginate and polyethylene glycol, dividing the suspension into bead-sized globules, contacting the globules with a solution of divalent cations to gel the alginate to form beads containing polyethylene glycol-filled areas and removing the polyethylene glycol to form voids in the beads entrapping the bioactive cells. By perfusing the beads with a nutrient medium, the cells can proliferate in the voids to produce high cell densities and produce a product which can be separated from the medium.
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Lehigh University
Naff David M.
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