Bio-synthetic photostimulators and methods of use

Details Bio-synthetic photostimulators and methods of use Bio-synthetic photostimulators and methods of use

2006-12-05

2006-12-05

Whiteman, Brian (Department: 1635)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

C514S703000

Reexamination Certificate

active

07144733

ABSTRACT:
Cells are rendered sensitive to stimulation by introducing into a non-photoreceptor cell nucleic acid sequences encoding at least an opsin gene product, an arrestin gene product, and the alpha subunit of the heterotrimeric G protein of the Gqfamily. The introduced sequences are expressed by the cell to yield at least the opsin gene product, the arrestin gene product, and the alpha subunit of the heterotrimeric G protein of the Gqfamily. Retinal or a derivative thereof capable of bonding with the opsin gene product to form a rhodopsin is provided to the cell. The cell is then irradiated with light having a wavelength capable of converting the rhodopsin to metarhodopsin. The conversion of rhodopsin to metarhodopsin triggers a cascade of intracellular responses within the cell resulting in an increased intracellular concentration of IP3and calcium ions.

REFERENCES:
patent: 2002/0123464 (2002-09-01), Kapeller-Libermann
patent: 2003/0013137 (2003-01-01), Barak et al.
Jindrova, Physiol. Res. 47: 155-168, 1998.
Pulvermuller, Biochemistry, 36:9253-9260, 1997.
Neves et al. Science, 295:1636-1639, 2002.
Anderson et al., Nature, vol. 392, pp. 25-30, 1998.
Verma, Nature, vol. 389, pp. 239-242, 1997.
Kmiec, American Scientist, 1999, 87, 240-247.
Juengst, BMJ, 2003, 326:1410-11.
Orkin et al., Dec. 7, 1995, “Report and Recommendation of the Panel to Assess the NIH investment in Research and Gene Therapy”, issued by the National Institute of Health.
Oakley et al. The Journal of Biological Chemistry 275:17201-17210, 2000.
Scott, K. et al.G q α Protein Function In Vivo: Genetic Dissection of Its Role in Photreceptor Cell Physiology: Neuron, vol. 15, Oct. 1995, pp. 919-927.
Chappell S.A., et al.A 9-nt Segment of a Cellular MRNA can Function as an Internal Ribosome Entry Site(IRES)and When Present in Linked Multiple Copies Greatly Enhances IRES Activity: Scripps Research Institute and Skaggs Institute for Chemical Biology., Feb. 2000, vol. 97, No. 4, pp. 1536-1541.
Bibikov, S. I. et al.Bacteriorhodopsin is involved in halobacterial Photoreception: Proc. Natural Acadamy Science, Oct. 1993, vol. 90, pp. 9446-9450.
Zemelman, B.V. et al.Selective Photostimulation of Genetically ChARGed Neurons: Neuron, Jan. 2002, vol. 33, pp. 15-22.

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