Binding reagents and methods

Chemistry: analytical and immunological testing – Biospecific ligand binding assay

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436518, 436529, 436543, 436547, 435 7, 435174, 435177, 530389, 530810, 530813, G01N 33543, G01N 33566

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active

047229063

ABSTRACT:
A method for selectively covalently linking a target moiety to a chemical moiety or carrier comprising attaching to the chemical moiety or carrier a reagent having a selector group capable of forming a specific bond with a receptor carried on the target moiety and attaching a latent reactive group which is capable upon activation of covalently bonding to the target moiety, reacting the selector group with the receptor on the target moiety, and activating the latent group to form a covalent linkage to the target moiety.

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patent: 4434150 (1984-02-01), Azad et al.
Guire, "Stepwise Thermophotochemical Crosslinking for Enzyme Stabilization and Immobilization", Enzyme Engineering 3, 63-70, (Plenum Publishing Corporation, 1978).
Friedberg, "Affinity Chromatography and Insoluble Enzymes", Chromatogr. Rev. 14, 121-131 (1971).
Green et al., "The Use of Fifunctional Biotinyl Compounds to Determine the Arrangement of Subunits in Avidin", Biochem J. 125, 781-791 (1971).
Gorman et al., "Transglutaminase Amine Substrates for Photochemical Labelling and Cleavable Cross-Linking of Proteins", J. Biol. Chem. 255, 1175-1180 (1980).
Darfler et al., "Applications of Light-Sensitive Chemicals for Probing Biological Processes", Chap. 2 of Chemistry and Biochemistry of Amino Acids, Peptides and Proteins.
Converse et al., Biochem., 8(11): 4431-4436 (1969).
Guire, Methods in Enzymology, VLIV, "Photochemical Immobilization of Enzymes and Other Biochemicals", Academic Press, N.Y., 280-288 (1976).
Chowdhry, Ann. Rev. Biochem., 48: 293-325 (1979).
Erecinska et al., Arch. Biochem. Biophys., 171: 108-116 (1975).
Katzenellenbogen et al., Biochem., 13(14): 2986-2994 (1974).

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