Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1984-08-06
1987-11-17
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
264 41, 4284022, 436 43, 436501, 436829, G01N 3353, G01N 33566, G01N 3500
Patent
active
047074418
ABSTRACT:
Disclosed is a specific binding assay composition and method for determining a ligand in a sample. The composition comprises (a) a binding partner for the ligand; (b) a detection system which has at least two components; (c) a selectively accessible vesicle having a surface-incorporated ligand or ligand analog and a first component of the detection system therein; (d) a substance which modifies vesicle accessibility in response to binding of surface-incorporated ligand or ligand analog and the binding partner; (e) at least one additional component of the detection system which is reactive with the first component to produce a detectable response; and (f) at least one surfactant which does not modify vesicle accessibility. The composition and method are suitable for use with automated, including continuous flow-type, analyzers.
REFERENCES:
patent: 4150949 (1979-04-01), Smith
patent: 4483921 (1984-11-01), Cole
Ahmad Syed I.
Hedaya Eddie
Greenman Jeffrey M.
Marantz Sidney
Technicon Instruments Corp.
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