Binding assays

Details Binding assays Binding assays

1997-08-07

2000-04-25

Caputa, Anthony C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 72, 435 78, 435 793, 435 5, 435 6, 4352351, 536 236, 536 2372, G01N 3353, C12Q 100, C12Q 170, C12N 701

Patent

active

060542810

ABSTRACT:
Disclosed is a method of testing a substance for the ability to affect the formation or oligomerization of a complex comprising members of a specific binding pair, a first member of the binding pair being present on the surface of a lipid enveloped particle comprising a transferrable label, said first member of the binding pair being capable of binding to a second member of the specific binding pair present on the surface of a cell, the lipid envelope of the particle being capable of fusing with the membrane of the cell so as to transfer the label to the cell, said transfer being inhibited by formation or oligomerization of a complex between the first and second members of the binding pair, wherein the method comprises reacting the particle and the cell, in the presence of the substance under test, (under conditions which would allow for binding of the first and second members of the specific binding pair in the absence of the substance under test), and detecting the label transferred, if any; together with a composition for use in the assay method of the invention.

REFERENCES:
patent: 5723287 (1998-03-01), Russell et al.
Cosset, et al, "Retroviral Retargeting By . . . " J. Virol. 69(10): 6314-6322, 1995.
Kasahara, et al, "Tissue-Specific targeting of . . . " Science 266:1373-1376, 1994.
Bevan et al., 1995, Identifying Small-molecule Lead Compounds: The Screening Approach To Drug Discovery, Trends in Biotechnology 13, p115-122.
Bosworth & Towers, 1989, Scintillation Proximity Assay, Nature 341, p167-168.
Cosset et al., 1995, Retroviral Retargeting by Envelopes Expressing an N-Terminal Binding Domain, J. Virol. 69, p6314-6322.
Ecker and Crooke, 1995, Combinatorial Drug Discovery: Which Methods Will Produce the Greatest Value? Bio/Technology 13, p351-360.
Hodgson J., 1992, Receptor Screening and the Search for New Pharmaceuticals, Bio/Technology 10, p973-980.
Luyten & Leysen, 1993, Receptor Cloning And Heterologous Expression-towards A New Tool For Drug Discovery, Biotechnology 11, p247-254.
Matano et al., 1995, Targeted Infection Of A Retrovirus Bearing A Cd4-env Chimera Into Human Cells Expressing Human Immunodeficiency Virus Type 1, Gen. Virol. 76, p3165-3169.
Matthews et al., 1994, A Survey Of Furin Substrate Specificity Using Substrate Phage Display, Protein Science 3, p1197-1205.
Matthews & Wells, 1993, Substrate Phage: Selection of Protease Substrates by Monovalent Phage Display, Science 260, p1113-1117.
Schertler, 1992, Overproduction Of Membrane Proteins, Curr. Opin. Struct. Biol. 2, p534-544.
Smith et al., 1995, Rapid Identification of Highly Active and Selective Substrates for Stromelysin and Matrilysin Using Bacteriophage Peptide Display Libraries, Biological Chemistry 270, p6440-6449.
Somia et al., 1995, Generation Of Targeted Retroviral Vectors By Using Single-chain Variable Fragment: An Approach To In Vivo Gene Delivery, Proc. Natl. Acad. Sci. 92, p7570-7574.
Valsesia-Wittman et al., 1994, Modifications in the Binding Domain of Avian Retrovirus Envelope Protein To Redirect the Host Range of Retroviral Vectors, J. Virol. 68, p4609-4619.

Affiliated with

Also associated with

Rating

Binding assays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Binding assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Binding assays will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-992289