Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1984-08-29
1986-12-16
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 6, 435 14, 435 18, 435803, 435807, 435810, 436 63, 436 94, 436501, 436537, 436541, 436825, 436808, 935 78, G01N 3353, G01N 33542
Patent
active
046296891
ABSTRACT:
At the conclusion of a selective binding assay (e.g., immunological or nucleic acid), an enzyme is present in modulated concentration and/or activity to moderate a chemical reaction such as the cleavage of o-nitrophenyl-.beta.-D-galactopyranoside. After a controlled period, the enzymatic product is transferred to the gas phase and concentrated relative to other components in the enzymatic reaction mixture, such as by extraction into ethyl ether, injection into a gas chromatography column and detection by flame ionization or electron capture. Kits for such assays are also disclosed.
REFERENCES:
patent: 4197369 (1980-04-01), Weaver
patent: 4322495 (1982-03-01), Kato
Vela, Clinical Chemistry, 14(8), pp. 837-838, (1968).
Hrivnak-Chemical Abstracts 72:28200A (1970).
Matsunaga-Chemical Abstracts 92:140189T (1980).
Diamond Steven E.
Regina Francis J.
Allied Corporation
Benson Robert
Doernberg Alan M.
Matthews Gale F.
Nucker Christine M.
LandOfFree
Binding assay with amplified read-out and gas-phase detection does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Binding assay with amplified read-out and gas-phase detection, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Binding assay with amplified read-out and gas-phase detection will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-245067