Binding assay system and method of making and using same

Details Binding assay system and method of making and using same Binding assay system and method of making and using same

1983-03-17

1986-01-28

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving iga, igd, ige, or igm

422 61, 422 68, 422 71, 435287, 436530, G01N 33053, G01N 2306, C12M 100, C01N 33544

Patent

active

045671494

ABSTRACT:
Method and apparatus for use in the diagnostic analysis of a liquid specimen through binding assays. The apparatus includes a rigid body having an elongated, shallow well formed in it, with a plurality of elongated strips, preferably in the form of cotton threads, stretched across the well in spaced relationship and generally perpendicular to the well's longitudinal axis. Each thread is coated with a separate binding conjugate such as an antigen or allergen. A cover plate that includes a thin plastic layer and an overlaying thin metallic layer covers the well to form an enclosed chamber having a specific volume. The metallic layer includes a separate elongated aperture in alignment with each coated thread. A liquid specimen is introduced into the chamber through one of two ports, for incubation with the coated threads, after which the specimen is removed and the reaction that occurred on each thread is determined.

REFERENCES:
patent: 3925116 (1975-12-01), Blume
patent: 4230757 (1980-10-01), Toner
patent: 4284725 (1981-08-01), Fennel, III et al.
patent: 4459360 (1984-07-01), Marinkovich

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