Binding assay for the detection of mycobacteria

Synthetic resins or natural rubbers -- part of the class 520 ser – Synthetic resins – Mixing of two or more solid polymers; mixing of solid...

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260112R, 424 11, 424 85, 424 87, 435272, 435863, 436534, 436808, 436811, C07G 700

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044106602

ABSTRACT:
A method for detecting the presence of Mycobacteria in a fluid or tissue which comprises mixing the fluid or tissue containing a secretory product of Mycobacteria with a complex of a tracer-containing molecule and a binding macromolecule having reversible binding affinity for the tracer-containing molecule and detecting the tracer-containing molecule, wherein the tracer-containing molecule is a charcoal-adsorbable protein from Mycobacterium tuberculosis which has a molecular weight of 20,000-30,000 and which is immunochemically stable from 4.degree.0 C. to 250.degree. C. and has a pH range from 3.0 to 9.0. The method is particularly applicable to the detection of infectious tuberculosis in humans and determining the antibiotic sensitivity of infecting Mycobacteria.

REFERENCES:
Gel Filtration Theory and Practice, Pharmacia Fine Chemicals, pp. 16-18.
"Radioimmunoassay for Tuberculin Purified Protein Derivative," E. Straus and R. Yalow, Clinical Research, vol. 25, No. 3, A384 Apr. 1977.
T. Daniel & P. Anderson, Am. Rev. Resp. Dis., vol. 117, 1978, pp. 533-539.
K. Gupta & S. Landi, Can. J. Microbiology, vol. 24, No. 10, 1978, pp. 1242-1249.

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