Binding assay employing a synthetic gene for D4 dopamine recepto

Details Binding assay employing a synthetic gene for D4 dopamine recepto Binding assay employing a synthetic gene for D4 dopamine recepto

1993-07-01

1995-11-21

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 691, 435 6, 536 235, 436501, C12N 1512

Patent

active

054686156

ABSTRACT:
A modified gene coding for the human D4 dopamine receptor has been synthesized by chemo-enzymatic methods. The nucleotide sequence of the D4 dopamine receptor gene was oh:raged to reduce the G+C content and to eliminate intronic sequences, while maintaining the published amino acid sequence. Using gene splicing by overlap extension and PCR amplification of long oligonucleotides (>200 bases), 3 synthetic fragments of about 400 base pairs each were amplified, from which the full length gene was assembled. Stable expression of this gene has been achieved in CHO-K1 cells, using an inducible expression system, and in HEK293 cells.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
VanTol, H. H. M., Bunzow, J. R., Guan, H.-C., Sunahara, R. K., Seeman, P., Niznik, H. B., and Civelli, O. (1991) Nature 350:610-614.
Mills, A., Allet, B., Bernard, A., Chabert, C., Brandt, E., Cavegn, C., Chollet, A. and E. Kawashima (1993) FEBS Lett. 320:130-134.
Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K., and Pease, L. R. (1989) Gene 77:61-68.
Sawadago, M. and VanDyke, M. W. (1991) Nucleic Acids Res. 19:674.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning-No Copy Enclosed.
Yates, J. L., Warren, N., and Sugden, B. (1985) Nature 313:812-815.
VanTol, H. H. M., Wu, C. M., Guan, H.-C., Ohhara, K., Bunzow, J. R., Civelli, Ol.,Kennedy, J., Seeman, Pl, Niznik, H. B., and Jovanovic, V. (1992) Nature 358:149-152.

Affiliated with

Also associated with

Rating

Binding assay employing a synthetic gene for D4 dopamine recepto does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Binding assay employing a synthetic gene for D4 dopamine recepto, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Binding assay employing a synthetic gene for D4 dopamine recepto will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1136168