Binary viral expression system in plants

Details Binary viral expression system in plants Binary viral expression system in plants

1998-10-23

2000-06-20

McElwain, Elizabeth F.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

435 691, 4353201, 435468, 800285, 800287, 800288, 800298, 800300, 800302, C12N 1582, C12N 1590, A01H 500, A01H 510

Patent

active

060779926

ABSTRACT:
This invention relates to a regulated binary plant viral expression system. It is comprised of two chromosomally-integrated components. One component is a proreplicon, which contains cis-acting viral sequences required for replication and a contains a target gene. The other component is a chimeric trans-acting replication gene comprising a regulated promoter operably-linked to the coding region for a viral replication protein. The proreplicon lacks the replication gene essential for replicon replication, and thus cannot undergo autonomous episomal replication. However, regulated expression of the trans-acting replication protein in plant cells also containing the proreplicon will trigger the release of free replicon from the integrated proreplicon, result in its episomal replication in trans, and result in the expression of the target gene, if present, through gene amplification. The expression system is useful for both production of foreign proteins as well as silencing endogenous genes and transgenes in plant tissue. Tissue-specific expression is controlled by the choice of promoter controlling the transcription of the trans-acting replication gene.

REFERENCES:
patent: 4855237 (1989-08-01), Morinaga et al.
Aryan et al, Mol. Gen. Genet., vol. 225, pp. 65-71, 1991
Vgacki et al, Nucl. Acids Res., vol. 19, pp. 371-377, 1991.
Topfer et al, Plant Cell, vol. 1, pp. 133-139, 1989.
Timmermans et al, Annu Rev. Plant Physiol. Mol. Biol., vol. 45, pp. 79-112, 1994.
Hennig et al, Plant J., vol.l 4, pp. 481-483, 1993.
Jach et al, Plant J., vol. 8, pp. 97-109, 1995.
Blockland et al, Plant J., vol. 6, pp. 861-877, 1994.
Hong et al., Resistance to geminivirus infection by virus-induced expression of dianthin in transgenic plants., Virology, (Jun. 1, 1996 220 (1) 119-27).
Hong et al., "Transactivation of dianthin transgene by African cassava mosaic virus AC2.", Virology, (Feb. 17, 1997 118 (2) 383-7).
Sablowski et al., Proc. Natl. Acad. Sci. USA, 92, 6901-6905, 1995.
Hayes et al., Nucleic Acids Res., 17, 2391-2403, 1989.
Hayes et al., Nature (London), 334, 179-182, 1988.
Rogers et al., Cell, 45, 593-600, 1986.
Goodman, J. Gen. Virol., 54, 9-21, 1981.
Hanley-Bowdoin et al., Plant Cell, 1, 1057-1067, 1989.
Hanley-Bowdoin et al., Proc. Natl. Acad. Sci. U.S.A., 87, 1446-1450, 1990.
Hayes et al., Nucleic Acids Res., 17, 10213-10222, 1989.
Al-Kaff et al., Science (Washington, DC), 279, 2113-2115, 1998.
Needham et al., Plant Cell Rep., 17, 631-639, 1998.
Senior et al., Biotechnol. Genet. Eng. Rev., 15, 79-119, 1998.
Thomas et al., Plant Growth Regul., 25, 205, 1998 abstracts.
Ruiz et al., Plant Cell, 10, 937-946, 1998.
Kjemtrup et al., Plant J., 14, 91-100, 1998.
Atkinson et al., Plant J., 15, 593-604.

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