Binary tumor therapy

Details Binary tumor therapy Binary tumor therapy

1991-10-11

1993-11-16

Nutter, Nathan M.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Heterocyclic carbon compounds containing a hetero ring...

514261, 514283, 514411, 514443, 514444, 514449, 514463, 514468, A01N 4300, A01N 4390, A61K 3133, A61K 3152

Patent

active

052624099

ABSTRACT:
Method for killing a cycling cell, by contacting the cell with a first agent that blocks progression of the cell cycle in the cell, and thereafter contacting the cell with a second agent that overrides the cell cycle block such that the cell proceeds past mitosis and cell death results within an additional cell cycle due to aberrant DNA replication or chromosome segregation. The first agent blocks the progression of the G.sub.1, S, G.sub.2, or mitosis stage of the cell cycle. The second agent is preferably 2-aminopurine (2-AP) or 6-dimethylaminopurine (6-DMAP). The duration of contact with the first agent is advantageously limited to a first time period sufficient to block the progression of the cell cycle, and the duration of contact with the second agent is limited to a second time period sufficient to override the cell cycle block. Also, a method of screening for a binary tumor therapy agent, by contacting a cycling mammalian cell with a first agent that blocks progression of the cell cycle, preferably mitosis, in the cell; thereafter contacting the cell with a candidate second agent; and determining that the candidate second agent is a binary tumor therapy agent if the candidate second agent overrides the cell cycle block such that the cell proceeds past mitosis and cell death results within an additional cell cycle due to aberrant DNA replication or chromosome segregation.

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