Binary probe and clamp composition

Details Binary probe and clamp composition Binary probe and clamp composition

2006-06-06

2006-06-06

Myers, Carla J. (Department: 1634)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S024300, C536S024310, C536S024320, C536S024330, C435S006120, C435S091100, C435S091200, C435S091500

Reexamination Certificate

active

07057025

ABSTRACT:
Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

REFERENCES:
patent: 4716106 (1987-12-01), Chiswell
patent: 4868105 (1989-09-01), Urdea et al.
patent: 5424413 (1995-06-01), Hogan et al.
patent: 5627032 (1997-05-01), Ulanovsky
patent: 5656731 (1997-08-01), Urdea
patent: 5681697 (1997-10-01), Urdea et al.
patent: 5723591 (1998-03-01), Livak et al.
patent: 5773571 (1998-06-01), Nielsen et al.
patent: 5801155 (1998-09-01), Kutyavin et al.
patent: 6030787 (2000-02-01), Livak et al.
patent: 6432642 (2002-08-01), Livak et al.
patent: 0 204 510 (1986-12-01), None
patent: 0 450 594 (1991-10-01), None
patent: 0 857 791 (1998-08-01), None
patent: 2 202 328 (1999-09-01), None
patent: WO 96/32496 (1996-10-01), None
Dueholm et al., “Synthesis of Peptide Nucleic Acid Monomers Containing the Four Natural Nucleobases: Thymine, Cytosine, Adenine, and Guanine and Their Oligomerization,”J. Org. Chem. 59:5767-5773 (1994).
Egholm et al., “Efficient pH-independent sequence-specific DNA binding by pseudoisocytosine-containing bis-PNA,”Nucleic Acids Research 23(2):217-222 (1995).
Gong et al., “New DNA Minor-Groove Binding Molecules with High Sequence-Selectivities and Binding Affinities,”Biochem. and Biophys. Res. Comm. 240:557-560 (1997).
Koch et al., “Improvements in automated PNA synthesis using Boc/Z monomers,”J. Peptide Res. 49:80-88 (1997).
Lukhtanov et al., “Oligodeoxyribonucleotides with Conjugated Dihydropyrroloindole Oligopeptides: Preparation and Hybridization Properties,”Bioconjugate Chem. 6:418-426 (1995).
Mullah et al., “Efficient synthesis of double dye-labeled oligodeoxyribonucleotide probes and their application in a real time PCR assay,”Nuc. Acids Res. 26(4):1026-1031 (1998).
van der Laan et al., “A Convenient Automated Solid-Phase Synthesis of PNA-(5′)-DNA-(3′)-PNA Chimera,”Tetrahedron Letters 38(13):2249-2252 (1997).
Vinayak et al., “Automated Chemical Synthesis of PNA and PNA-DNA Chimera on a Nucleic Acid Synthesizer,”Nucleosides&Nucleotides 16(7-9):1653-1656 (1997).
Matthews et al.,Analytical Biochemistry169:1-25 (1998).
Livak, K.J., et al., “Oligonucleotides with Fluorescent Dyes at Opposite Ends Provide a Quenched Probe System Useful for Detecting PCR Product and Nucleic Acid Hybridization,”Genome Research 4(6):357-362 (1995).
Afonina, et al., “Efficient Priming of PCR With Short Oligonucleotides Conjugated to a Minor Groove Binder,”Nucleic Acids Research 25(13):2657-2660 (1997).
Zelphati, O. et al., “PNA-Dependent Gene Chemistry: Stable Coupling of Peptides and Oligonucleotides to Plasmid DNA,”BioTechniques28:304-316 (Feb. 2000).

Affiliated with

Also associated with

Rating

Binary probe and clamp composition does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Binary probe and clamp composition, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Binary probe and clamp composition will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3683763