Binary kinetic assay method and apparatus

Chemistry: analytical and immunological testing – Rate of reaction determination

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

422 68, 435 4, 436164, G01N 2177, G01N 3300

Patent

active

045392951

ABSTRACT:
A kinetic assay method and apparatus for performing such method wherein a first sample containing predetermined concentrations of binary analytes is reacted with the reagent to form a reaction product. Reaction rates at first and second times are measured for the first reaction and a first ratio between such measured reaction rates is determined. A second reaction with a second sample and the reagent is also performed wherein the second sample contains predetermined concentrations of the binary analytes. Reaction rates at the first and second times are measured for the second reaction and a second ratio between such rates is determined. A third reaction with a third sample and the reagent is performed, the sample including unknown relative concentrations of the first and second analytes. Reaction rates are again measured at the first and second times and a third ratio between such measured reaction rates is determined.
By comparing the first and third ratios, an indication can be provided to show that the reaction is not influenced by an interfering analyte. The third ratio may be compared to a range of ratios defined by the first and second ratios to determine the relative contribution of at least one of the analytes to a reaction product formed during the third reaction. The relative contribution of a selected one of the analytes may be used to determine a concentration of such analyte corrected for the influence or bias of the other analyte in the reaction.

REFERENCES:
patent: 3378463 (1968-04-01), Guilbault et al.
patent: 3682586 (1972-08-01), Ertinghausen et al.
patent: 3857771 (1974-12-01), Sternberg
patent: 3881992 (1975-05-01), Ralston
patent: 3894843 (1975-07-01), Jarvis
patent: 3915644 (1975-10-01), Walraven
patent: 3989383 (1976-11-01), Paulson
patent: 4111657 (1978-09-01), Denney et al.
patent: 4289498 (1981-09-01), Baughman et al.
patent: 4309112 (1982-01-01), Ashley et al.
Fabiny, D. L. and Ertingshausen, G., "Automated Reaction-Rate Method for Determination of Serum Creatinine with the CentrifiChem," Clinical Chemistry, vol. 17, No. 8, 1971, pp. 696-700.
Bowers, L. D., "Kinetic Serum Creatinine Assays I. The Role of Various Factors in Determining Specificity," Clinical Chemistry, vol. 26, No. 5, 1980, pp. 551-554.
Bowers, L. D. and Wong, E. T., "Kinetic Serum Creatinine Assays. II. Critical Evaluation and Review," Clinical Chemistry, vol. 26, No. 5, 1980, pp. 555-561.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Binary kinetic assay method and apparatus does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Binary kinetic assay method and apparatus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Binary kinetic assay method and apparatus will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-613676

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.