Bilirubin determination

Chemistry: physical processes – Physical processes – Crystallization

Patent

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Details

252408, G01N 3316

Patent

active

040308855

ABSTRACT:
An improved azobilirubin determination procedure utilizes a stable, dry lyophilized reagent. Dissolving the dry reagent in dilute hydrochloric acid produces a diazo reagent solution which is stable for several days. Cysteine hydrochloride may be added to the assay mixture to destroy excess diazo reagent, stabilize the final color, and remove interfering metal ions.

REFERENCES:
patent: 2737501 (1956-03-01), Sherman
patent: 3511607 (1970-05-01), Green
patent: 3652222 (1972-03-01), Denney et al.
patent: 3825411 (1974-07-01), Morin
patent: 3955926 (1976-05-01), Fischer

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