Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1996-11-27
1998-09-08
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, 435 4, 435975, 436 66, 436 63, 436 68, 436 97, 562 58, 422 50, 422 681, 422 61, 422 62, C12Q 128, C12Q 126, C12Q 100, G01N 3353
Patent
active
058044051
ABSTRACT:
A method is taught by which a sample can be analyzed for the selective determination of the total concentration of bilirubin (B.sub.t), the concentration of conjugated bilirubin (B.sub.c), the concentration of unconjugated bilirubin (B.sub.u), the concentration of unbound bilirubin (b), the concentration of unbound, unconjugated bilirubin (b.sub.u) using a unique combination of enzymatic and colorimetric methods in a single assay, so that the potentially neurotoxic, non-albumin bound, unconjugated fraction of total bilirubin in the sample (i.e., b.sub.u) can be measured in a simple, quick and accurate manner. A kit utilizing the same assay is also described. Both kit and methods will be of use to the clinician and general researcher. The B.sub.t, B.sup.c, B.sub.u, b and b.sub.u are determined using spectra, absorption or emission characteristics in combination with kinetic measurements of rates of reaction of chemical processes.
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Leary Louise N.
Research Corporation Technologies Inc.
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