Bilirubin assay

Details Bilirubin assay Bilirubin assay

1982-02-22

1983-09-13

Turk, Arnold

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-n

422 61, 436 71, 436175, 436903, G01N 3372, G01N 3392

Patent

active

044042868

ABSTRACT:
An improved reagent and method for assay of total bilirubin in biological fluids utilizes diazo sulfanilic acid to convert bilirubin to azobilirubin which is then quantitatively measured. The improved reagent and method include the solvent accelerator tetramethylene sulfone. The use of tetramethylene sulfone as also allows the use of nonionic surfactants, obviating the need for serum blank correction in highly lipemic serum. Furthermore, the use of tetramethylene sulfone and nonionic surfactants renders the reagent mixture less viscous facilitating automated analysis.

REFERENCES:
patent: 2737501 (1956-03-01), Sherman
patent: 3853473 (1974-12-01), Morin et al.
patent: 4030885 (1977-06-01), Das
patent: 4078892 (1978-03-01), Steinbrink, Jr.
patent: 4282001 (1981-08-01), Klose et al.
patent: 4311483 (1982-01-01), Perry

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