Bifunctional protein from carrots (Daucus carota) with aspartoki

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435183, 435194, C12N 904, C12N 900, C12N 912

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active

058587497

ABSTRACT:
A bifunctional enzyme has been isolated and purified from carrots which has both aspartokinase and homoserine dehydrogenase activities in the same polypeptide. The enzyme can be used to regulate lysine, homoserine, threonine, isoleucine and methionine content in plants. The DNA sequence encoding the bifunctional enzyme is also disclosed.

REFERENCES:
patent: 5451516 (1995-09-01), Matthews et al.
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Wilson et al. (1991) Plant Physiol., 97(4), "Bifunctional Protein in Carrot Contains Both Aspartokinase and Homoserine Dehydrogenase Activies", pp. 1323-1328.
Aarnes et al. (1974) Phytochemistry, 13(12), "Threonine-Sensitive Aspartate Kinase and Homoserine Dehydrogenase from Pisum sativum", pp. 2717-2724, in Chem. Abstr., 82, p. 178, Abstr. No. 108,005.
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