Bifunctional DNA-protein conjugating agent

Details Bifunctional DNA-protein conjugating agent Bifunctional DNA-protein conjugating agent

1986-03-13

1989-10-10

Brown, Johnnie R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 14, 435 25, 435188, 435803, 935 78, C12Q 168, C12Q 170, C12Q 154, C12N 996

Patent

active

048731870

ABSTRACT:
A cationic detergent having a positively charged group and a hydrophobic group is used to conjugate an enzymatically active enzyme molecule with a single-stranded nucleic acid molecule. The conjugate may be used to detect the presence of nucleic acid molecules having a nucleotide sequence which is complementary to that of the single-stranded molecule of the conjugate.

REFERENCES:
patent: 4055469 (1977-10-01), Snoke et al.
patent: 4235759 (1980-11-01), Ohbu et al.
patent: 4454060 (1984-06-01), Lai et al.
Geck, P. et al., Anal. Biochem., 135 (1983), p. 264-268.
Chem. Abst. 92, No. 1, Issued Jan. 7, 1980, p. 279, 2787k, Khristova, M. L. et al., "Use of the Cationic Detergent . . . RNA".
Renz, M. et al., Nuckic Acids Research, 12 (1984), pp. 3435-3444.

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